A overview of the tools and methods used to assess eel presence, environmental suitability, and population health through integrated monitoring approaches.
Eels (tuna) are highly adaptable freshwater species, but their distribution, health, and abundance are still strongly influenced by water quality, habitat condition, and catchment pressures. To assess eel presence and environmental suitability, a combination of physical water quality measurements, molecular detection, biological examination, and active capture techniques was used. The equipment included a water quality probe, environmental DNA (eDNA) sampling, a dissection kit, Mauri Compass App, and a fyke net (hīnaki). Each method provides distinct but complementary information, improving confidence in results and interpretation.
The probe was calibrated prior to use and then placed into flowing sections of the stream at mid-depth to avoid surface warming effects and sediment disturbance. Readings were allowed to stabilise before recording temperature, pH, dissolved oxygen (DO), and conductivity. Measurements were taken at multiple points to account for small-scale variation in water chemistry.
Water quality strongly influences eel physiology and habitat suitability. While eels are more tolerant than many native fish species, prolonged exposure to poor water quality can reduce growth rates, increase stress, and limit successful migration. Measuring these parameters allows environmental conditions to be linked directly to eel presence, size, and condition.


Optimal range for eels: ~6.5–8.0
Poor / stressful: < 6.0 (acidic) or > 8.5 (alkaline)
Low pH can damage gill tissue and disrupt ion balance, while high pH can increase ammonia toxicity.
Healthy freshwater range: ~50–300 µS/cm
Elevated / poor: > 400–500 µS/cm
High conductivity often indicates nutrient enrichment, urban runoff, or salinity intrusion, which can degrade eel habitat quality.
Good: > 6 mg/L
Poor: < 4 mg/L
Although eels can tolerate low oxygen better than most fish, persistently low DO increases stress and reduces activity.
Water temperature was measured using the water quality probe, which was placed at mid-depth in flowing water and allowed to stabilise before recording. Temperature was measured alongside other water quality variables for consistency.
Temperature influences eel metabolism, activity, and growth, as eels rely on surrounding water to regulate body processes. It also affects dissolved oxygen levels and food availability.
Water temperature helps indicate whether a stream provides suitable conditions for eel feeding, breathing,movement, and long-term habitat quality.
Together, these measurements indicate whether the stream provides a chemically suitable environment for sustaining eel populations and help identify possible catchment scale impacts.
The fyke net (hīnaki) was set overnight with the entrance facing downstream or perpendicular, positioned along natural eel movement pathways such as stream margins and deeper channels. Nets were checked promptly to minimise stress. Captured eels were identified to species, measured for length, and assessed for condition before release.
Fyke nets are effective because they exploit eel movement behaviour rather than relying on attraction or bait. The hīnaki also reflects long-standing Māori knowledge of eel ecology, reinforcing culturally informed monitoring practices.


The dissection kit was used to carefully examine eel specimens under controlled conditions. External features such as body condition, skin integrity, and fin damage were recorded. Internal examination focused on the digestive tract and major organs to assess feeding status and overall health.
Indicates recent feeding success and available prey
Reflect nutritional status and habitat quality
May indicate environmental stress or degraded conditions
Supports understanding of whether the habitat is used for growth, residency, or migration
Dissection allows direct observation of biological indicators that reflect both environmental quality and eel life history. Internal condition can reveal long-term stressors that are not immediately apparent from water quality data alone.

Alternative method – Ice
An alternative humane method is placing the eel into an ice slurry (a mix of ice and water), which gradually lowers body temperature and slows metabolism. This reduces activity and responsiveness, leading to loss of consciousness with minimal stress when performed correctly.
Clove oil (eugenol) was prepared as a dilute solution and added to a container of stream water. Eels were gently placed into the solution and monitored closely. Within a short period, the eel became sedated and unresponsive to external stimuli, indicating loss of consciousness. For euthanasia, the eel remained in the solution for a sufficient duration to ensure death before any dissection occurred. Throughout the process, handling was minimised and monitoring was continuous to ensure the procedure was humane and effective.
Clove oil is a widely accepted, humane anaesthetic for fish and eels. It is effective at low concentrations, acts quickly, and causes minimal distress when used correctly. Using clove oil aligns with ethical research practices, animal welfare guidelines, and kaupapa Māori principles of respect for living organisms. Humane handling ensures that data collected reflects true environmental and biological conditions rather than stress responses caused by poor handling.


Water samples were collected upstream of physical sampling areas to avoid contamination from captured or handled eels. Sterile bottles were used, and samples were sealed immediately. Samples were filtered and analysed in a laboratory to amplify eel-specific DNA markers.
eDNA is particularly effective for detecting species that are elusive, nocturnal, or present at low densities. It complements physical capture methods by reducing sampling bias and eliminating the need to disturb habitat or organisms during detection.



The Mauri Compass App was used in the field to record all observations, measurements, and monitoring results in real time. Data collected from the water quality probe, eDNA sampling, fyke netting, and visual site observations were entered directly into the app during the assessment. The app guided the assessment process by prompting users to consider multiple environmental attributes rather than focusing on a single indicator.
The Mauri Compass App is designed to support holistic environmental assessment grounded in Te Ao Māori. Rather than relying solely on numerical data, the app integrates scientific measurements with cultural and observational indicators. This ensures that decision making reflects not just physical water quality, but also ecosystem health, mahinga kai values, and the wellbeing of people connected to the site.
Using the app in real time reduces data loss, improves accuracy, and allows immediate reflection on site conditions. It also encourages consistent assessment between sites and observers.
The Mauri Compass App helps interpret environmental data within a holistic framework of mauri . By combining quantitative measurements with qualitative observations, the app provides an overall indication of ecosystem health.
Specifically, it helps to:

Water clarity was measured using a clarity tube. Stream water was poured into the tube until the black-and-white target at the base became visible. The depth at which the target could be seen was recorded as the clarity measurement.
Clarity is an important indicator of sediment levels and disturbance within the catchment. While eels are tolerant of lower clarity than many fish species, excessive suspended sediment can smother habitat, reduce invertebrate abundance, and affect gill function.
Low clarity often reflects upstream erosion, stock access, or runoff following rainfall. Reduced clarity can limit prey availability and degrade benthic habitat used by eels for shelter and feeding.

Stream velocity was measured by marking a 10-metre section of the stream. A floating object such as a lemon or orange was released at the upstream point and the time taken to travel the 10 metres was recorded using a stopwatch. This was repeated multiple times and an average time was calculated. Velocity was then estimated using the formula:
Velocity = Distance ÷ Time
Stream velocity influences eel movement, energy expenditure, and habitat use. Eels prefer slow to moderate flows where they can move efficiently, shelter behind structures, and forage effectively. Velocity also affects sediment transport and channel stability.
Velocity measurements help explain eel distribution within the stream and provide context for fyke net placement and capture success.
Conclusion
Using clearly defined water quality thresholds alongside biological and molecular monitoring methods provides a strong, integrated understanding of eel habitat suitability and population condition. The probe identifies chemical stressors, eDNA confirms presence without disturbance, dissection reveals biological health, and fyke nets provide direct population data. The Mauri Compass App played a critical role in integrating these findings into a holistic assessment framework. Together, these methods allow robust interpretation of eel ecology and freshwater system health, supporting effective management and conservation.
Mauri Compass Equipment Used for Eel (Tuna) Monitoring